Specimen Collection of Fungal Keratitis

Standard procedures followed for ocular specimen collection, culture isolation and identification of the organisms are described below., Specimen Collection of Fungal Keratitis, CLINICAL ATLAS,

Fig. 1: Materials used for collecting specimens from corneal ulcers

Figs 2A and B: Corneal scraping collected by an ophthalmologist by using slit lamp microscope

Fig. 3 : Corneal biopsy collected by ophthalmologist in an operation theater

Figs 4A and B: Corneal scraping collected by an ophthalmologist by using ophthalmic microscope

Figs 4C and D: Infected area gently scraped by using Kimura’s spatula.
Scrapping helps to debulk the fungal load as in this case

Figs 4E and F: Scraped material inoculated on Blood agar in multiple
C streaks form (E) and smeared on a clean glass slide (F)