[Microbiology] Atlas of Direct examination in selected Gram-negative bacillary infections

Direct examination in selected Gram-negative bacillary infections, P. aeruginosa, Fusobacterium nucleatum, Bacteroides spp, Legionella pneumophila, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Atlas of Direct examination in selected Gram-negative bacillary infections, atlas in medical, tuyenlab.net

Fig 1.  Cerebrospinal fluid, drop smear, Gram stain,
light microscopy, HPV. Purulence, moderate. Gram-negative
coccobacilli, chains. Morphotype suggests Bacteroides spp.
Impression: gram-negative bacillary meningitis.

Fig 2.  Amniotic fluid, cytocentrifuge preparation, Gram
stain, light microscopy, HPV. Purulence, moderate. Local
materials, moderate. Gram-negative coccobacilli. Gramnegative bacilli,
filamentous, medium, fusiform. Morphology
suggests gram-negative bacillary anaerobic infection.
Impression: amnionitis, mixed anaerobic bacteria.

Fig 3.  Bronchoalveolar lavage, cytocentrifuge
preparation, Gram stain, light microscopy, HPV. Purulence,
moderate. Local materials, light. Gram-negative bacilli, small,
intracellular within phagocytic vacuoles (see Fig 4).

Fig 4.  Bronchoalveolar lavage, cytocentrifuge
preparation, direct fluorescent antibody (DFA). Legionella
pneumophila, polyvalent antisera, fluorescent microscopy,
HPV. Immunofluorescence positive. Impression: Legionnaires’
disease.

Fig 5.  Expectorated sputum, smear, Gram stain, light
microscopy, MPV. Purulence, light. Local materials, light.
Mucus moderate. Gram-negative bacilli, medium. Impression:
enteric bacillary infection.

Fig 6.  Urine, direct drop smear, Gram stain, light
microscopy, MPV. Purulence, heavy. Gram-negative bacilli,
medium. Gram-positive cocci. Urine culture grew Escherichia
coli and Enterococcus faecalis. The smear is consistent with a
bacterial density of 105 colony-forming units (CFU) per
milliliter of urine.

Fig 7.  Decubitus skin ulcer, smear, Gram stain, light
microscopy, HPV. Purulence, moderate. Amorphous debris,
heavy. Gram-negative bacilli, medium, encapsulated. Yeast.
Morphology suggests Klebsiella pneumoniae. Impression:
enteric bacillary disease.

Fig 8.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, moderate. Mucus, moderate.
Gram-negative bacilli, aberrant, encapsulated. Morphology
suggests antibiotic-affected Klebsiella pneumoniae.
Impression: enteric bacillary disease, partially treated.

Fig 9.  Peripheral blood, smear, Wright-Giemsa stain,
light microscopy MPV. Bacilli, medium, bipolar staining
(see Fig 10).

Fig 10.  Peripheral blood, smear, Gram stain, light
microscopy, HPV. Local materials, moderate. Gram-negative
bacillus, medium, with prominent bipolar staining. Suspicious
for Yersinia pestis. Impression: bubonic plague.

Fig 11.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, none. Local materials, none.
Mucus present. Gram-negative bacilli, regular, chains.
Morphotype suggests Pseudomonas aeruginosa. Impression:
pseudomonas infectious disease.

Fig 12.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, light. Local materials, none.
Mucus present. Gram-negative bacilli, regular, enveloped
in prominent slime layer. Morphotype suggests mucoid
P. aeruginosa. Impression: pseudomonas infectious disease.

Fig 13.  Amniotic fluid, cytocentrifuge preparation, Gram
stain, light microscopy, HPV. Purulence, light. Local material,
moderate. Gram-negative bacilli, fusiform. Morphology
suggests Fusobacterium nucleatum. Impression: amnionitis
anaerobic bacteria.

Fig 14. Amniotic fluid, cytocentrifuge preparation, Gram
stain, light microscopy, HPV. Purulence, moderate. Local
material, moderate. Gram-negative bacilli, medium and long
forms. Morphology suggests Fusobacterium spp. Impression:
amnionitis, anaerobic bacteria.

Fig 15.  Colonies on blood agar medium, subculture from
blood culture, smear, Gram stain, light microscopy, HPV.
Local material, light. Gram-negative bacilli with spirals, gull
wings. Morphology suggests Campylobacter spp.

Fig 16. Amniotic fluid, drop smear, Gram stain, light
microscopy, HPV. Purulence, moderate. Local materials,
moderate. Gram-negative bacilli, spiral. Impression:
amnionitis.

This is a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: http://amzn.to/2ctxo02