[Microbiology] Atlas of Direct examination in selected Gram-negative Bacterial infections

Direct examination in selected Gram-negative Bacterial infections, Moraxella catarrhalis, Haemophilus influenzae, Bordetella pertussis, Neisseria meningitidis, atlas in medical, tuyenlab.net

Fig 1.  Expectorated sputum, smear, Gram stain, light
microscopy, MPV. Mixed materials, type I, layered.
Contaminating bacteria and epithelial cells, moderate.
Purulence, light. Gram-negative diplococci. Morphology
suggests pathogenic Neisseria or Moraxella spp. Routine
bacterial culture isolated Moraxella catarrhalis.

Fig 2.  Expectorated sputum, smear, Gram stain, light
microscopy, MPV. Purulence, moderate. Local materials,
moderate. Gram-negative diplococci, intracellular,
extracellular. Morphology suggests pathogenic Neisseria or
Moraxella spp. Routine bacterial culture isolated Neisseria
meningitidis.

Fig 3.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, moderate. Local materials,
heavy. Mucus present. Gram-negative coccobacilli,
intracellular, extracellular. Morphology consistent with
Haemophilus influenzae.

Fig 4.  xpectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, heavy. Local materials,
moderate. Gram-negative bacilli, small, pleomorphic,
intracellular, extracellular. Gram-positive diplococci,
encapsulated, intracellular, extracellular. Morphology
consistent with H. influenzae and S. pneumoniae.
Impression: polymicrobial infection.

Fig 5.  Bronchoalveolar lavage, cytocentrifuge
preparation, Wright-Giemsa stain, light microscopy, HPV.
Purulence, none. Lymphocytes present. Local materials,
moderate. Small bacilli, numerous (see Fig 6).

Fig 6.  Bronchoalveolar lavage, cytocentrifuge
preparation, Gram stain, light microscopy, HPV. Purulence,
none. Lymphocytes present. Local materials, moderate.
Gram-negative bacilli, small (see Fig 7).

Fig 7.  Bronchoalveolar lavage, cytocentrifuge
preparation, Wright-Giemsa stain, light microscopy, HPV.
Purulence, none. Lymphocytes present. Local materials,
moderate. Gram-negative bacilli, small (see Fig 8).

Fig 8.  Bronchoalveolar lavage, cytocentrifuge
preparation, Wright-Giemsa stain, light microscopy, HPV.
Purulence, none. Lymphocytes present. Local materials,
moderate. Ciliated columnar epithelial cells with numerous
small bacilli adherent to cilia. Morphology consistent with
Bordetella pertussis. Impression: whooping cough.

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