[Microbiology] Atlas of Direct examination in Parasitic infections

Direct examination in Parasitic infections, Acanthamoeba spp, Toxoplasma gondii, Pneumocystis jiroveci, Enterocytozoon bieneusi, Cyclospora, Cryptosporidium parvum,Strongyloides stercoralis, Phthirus pubis, Trichinella spiralis, atlas in medical, tuyenlab.net, Atlas of Direct examination in Parasitic infections

Fig 1.  Cornea, scraping, Wright-Giemsa stain, light
microscopy, HPV. Purulence, none. Local materials,
moderate. Parasitic precyst (13 µm). Morphology consistent
with Acanthamoeba spp.

Fig 2.  Cornea, scraping from Plate 91, calcofluor white
stain, fluorescence microscopy, MPV. Polyhedral parasitic cyst
(13 µm). Morphology consistent with Acanthamoeba cyst.
Impression: Acanthamoeba keratitis.

Fig 3.  BAL, cytocentrifuge preparation, Wright-Giemsa
stain, light microscopy, HPV. Purulence, light. Local materials,
light. Amorphous debris light. Crescent-shaped cells with
central nucleus (see Fig 4).

Fig 4.  BAL, cytocentrifuge preparation, acridine orange
stain, fluorescence microscopy, HPV. Crescent-shaped cells
composed of RNA. Morphology consistent with trophozoites
(tachyzoites) of Toxoplasma gondii. Impression:
toxoplasmosis.

Fig 5.  BAL, cytocentrifuge preparation, Gram stain,
light microscopy, HPV. Purulence, none. Local materials,
moderate. Alveolar cast composed of gram-negative matrix
and intracystic bodies. Morphology consistent with
Pneumocystis jiroveci (carinii) (see Fig 6).

Fig 6.  BAL, cytocentrifuge preparation, calcofluor white
stain, fluorescence microscopy, HPV. Fluorescent cysts with
coccoid bodies. Morphology consistent with P. jiroveci
(carinii). Impression: pneumocystosis.

Fig 7.  Diarrheic stool, smear, modified Weber stain,
light microscopy, 2000× oil. Purulence, none. Parasite spores,
small (1.5 × 0.9 µm). Morphology consistent with
enterocytozoon (arrow 1) (see Fig 8). Compare with size
of the yeast (arrow 2).

Fig 8.  Diarrheic stool from concentration, transmission
electron microscopy, 57,000×. Endospore layer and polar
tubes present. Morphology consistent with Enterocytozoon
bieneusi. Impression: intestinal microsporidiosis.

Fig 9.  Watery, frothy diarrheic stool, smear, acid-fast
stain, MPV. Purulence, none. Local materials, heavy. Acid-fast
oocysts (4 to 6 µm) (see Plate 100). The measurement is
taken to clearly separate this oocyst from the 8 to 10 µm
oocysts of Cyclospora spp.

Fig 10.  Watery, frothy diarrheic stool from Plate 99,
smear, acid-fast stain, HPV. Acid-fast oocysts (4 to 6 µm).
Sporulated oocysts containing four sporozoites. Morphology
consistent with Cryptosporidium parvum. Impression:
cryptosporidiosis.

Fig 11.  Five-percent sheep blood agar plate inoculated
with expectorated sputum, 24-hour incubation with 5% CO2
in air. Note the heavy bacterial growth in the area of
primary inoculation with thin trails of colonies lacing the
surface of the agar (see Fig 12).

Fig 12.  Aspirated sputum, Gram stain, light microscopy,
LPV. Purulence, none. Local materials, moderate. Mucus
present. Coiled nematode larvae. Morphology consistent
with Strongyloides stercoralis. Impression: Strongyloides
hyperinfestation syndrome.

Fig 13.  Parasite, pubic hair from surgical patient,
unstained mount, light microscopy, LPV. Three pairs of legs
are identified with characteristic claws at tips. Morphology
consistent with Phthirus pubis (crab louse). Impression: louse
infestation.

Fig 14.  Muscle tissue, directly viewed. Encysted calcified
larvae. Morphology consistent with Trichinella spiralis.
Impression: trichinosis.

This is a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: http://amzn.to/2ctxo02