[Microbiology] Atlas of Direct examination in fungal infections

Direct examination in fungal infections, Histoplasma capsulatum, Blastomyces dermatitidis, Aspergilus spp, Cryptococcus neoformans, Cryptococcus, Candida spp, MICROBIOLOGY ATLAS, SUBCLINICAL ATLAS, atlas in medical, tuyenlab.net

Fig 1.  BAL, cytocentrifuge preparation, Gram stain,
microscopy, HPV. Purulence, light. Local materials, moderate.
Gram-positive yeast with buds. Morphotype consistent with
Candida spp. Impression: candidiasis.

Fig 2.  Expectorated sputum smear, Gram stain, light
microscopy, HPV. Purulence, light. Local materials, moderate.
Gram-positive pseudohyphae. Morphotype consistent with
Candida spp. Impression: candidiasis.

Fig 3.  BAL, cytocentrifuge preparation, Gram stain,
light microscopy, HPV. Local materials, moderate. Red blood
cells present. Gram-variable yeast with capsules. Morphology
suggests Cryptococcus (see Fig 4).

Fig 4.  BAL, cytocentrifuge preparation, calcofluor-white
stain, fluorescence microscopy, HPV. Fluorescent yeast, small,
with capsules. Morphology suggests Cryptococcus
neoformans. Impression: cryptococcosis.

Fig 5.  BAL, cytocentrifuge preparation, calcofluor white
stain, light microscopy, HPV. Fluorescent yeast (2 to 4 µm),
small, budding. Morphology suggests Histoplasma
capsulatum. Impression: histoplasmosis.

Fig 6.  Expectorated sputum, smear, calcofluor white
stain, fluorescence microscopy, MPV. Yeast (8 to 20 µm),
round, thick-walled, broad-based bud. Morphology suggests
Blastomyces dermatitidis. Impression: blastomycosis.

Fig 7.  Expectorated sputum, smear, calcofluor white
stain, fluorescence microscopy, MPV. Eosinophils. These cells
are another component that stains with calcofluor white.
The granules from ruptured eosinophils stain brightly and
should not be interpreted as remnants of fungi or parasites.

Fig 8.  Skin scales, scrapings, potassium hydroxide (KOH)
wet preparation, light microscopy, HPV. Hyphae present,
septate, thin. Morphology suggests dermatophyte.

Fig 9.  Skin scales from scrapings, calcofluor white stain,
fluorescence microscopy, HPV. Hyphae, thin. Morphology
suggests dermatophyte. Impression: dermatophytosis.

Fig 10.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, light. Local materials, heavy.
Gram-variable hyphae present (3 to 10 µm), septate,
branched 45-degree angle. Morphology suggests Aspergilus
spp. (see Fig 11).

Fig 11.  Expectorated sputum, smear, calcofluor white
stain, fluorescence microscopy, MPV. Fungal hyphae present
(3 to 10 µm), septate, branched 45-degree angle.
Morphology suggests Aspergilus spp. Impression:
aspergillosis.

Fig 12.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, none. Local materials,
moderate. Gram-positive conidia (2 to 4 µm), in chain.
Morphotype suggests Aspergilus spp. in cavity with air
interface. Impression: cavitary aspergillosis. Care must be
taken not to mistake these conidia for streptococci or for
yeast (see Fig 13).

Fig 13.  Expectorated sputum, smear, Gram stain, light
microscopy, HPV. Purulence, none. Local materials,
moderate. Gram-negative conidia (2 to 4 µm), sporulating.
Care must be taken not to confuse these conidia with yeast
germ tubes.

Fig 14.  Brain abscess, smear, toluidine blue stain, light
microscopy, MPV. Fungal hyphae present (3 to 10 µm),
septate, branched 45-degree angle. Morphology suggests
Aspergilus spp. Impression: cerebral aspergillosis.

Fig 15.  Soft tissue abscess, smear, calcofluor white stain,
fluorescence microscopy, HPV. Fungal hyphae, septate,
branched chlamydospores. Impression: Mycosis.
These hyphae were not clearly visible on the Gram stain
smear but stain brightly here. A dermatophyte was isolated
in culture.

This is a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: http://amzn.to/2ctxo02