[Microbiology] Atlas of Direct examination in Viral infections

Direct examination in Viral infections, Atlas of Direct examination in Viral infections, MICROBIOLOGY ATLAS, SUBCLINICAL ATLAS, atlas in medical, tuyenlsb.net

Fig 1.  Skin, vesicle fluid, Tzanck preparation,
hematoxylin and eosin (H&E) stain, light microscopy, MPV.
Purulence, moderate. Local materials, light. Multinucleated
epithelial cells present. Intranuclear inclusions present.
Morphology consistent with herpes viral inclusions.
Impression: herpes simplex infection.

Fig 2.  BAL, cytocentrifuge preparation, rapid WrightGiemsa stain,
light microscopy, MPV. Purulence, light. Local
materials, light. Red blood cells present. Multinucleated
epithelial cells present. Intranuclear inclusions present.
Morphology consistent with herpes viral inclusions.
Impression: herpes simplex infection. Compare with Plate
105. Note the change in appearance of the herpes-infected
cells with the change in the type of fixation and stain. The
H&E stain more clearly shows the “ground-glass” appearance
of the nuclear inclusion rimmed by the cell nuclear
chromatin. The rapid Wright’s stain provides an adequate
visual presentation and is more time efficient.

Fig 3.  Skin, vesicle fluid, Tzanck preparation, WrightGiemsa stain,
light microscopy, HPV. Purulence, light.
Multinucleated epithelial cells present. Intranuclear
inclusions present. Morphology consistent with herpes viral
inclusions. Impression: varicella-zoster infection.

Fig 4.  Skin, vesicle fluid, Tzanck preparation, antibody
stain for herpes simplex virus, fluorescent microscopy, MPV.
Immunostaining positive. Herpes simplex infection,
confirmed.

Fig 5.  BAL, cytocentrifuge preparation, Wright-Giemsa
stain, light microscopy, MPV. Purulence, light. Blood,
moderate. Local materials, light. Enlarged pneumocyte with
intranuclear inclusion. Morphology consistent with
cytomegalovirus. Observe the characteristic nuclear changes
for cytomegalovirus. The cell and the nucleus are enlarged,
the nucleus is granular, and the nuclear membrane is
indistinct. Blood is an indication of capillary damage.
Impression: cytomegalovirus disease.

Fig 6.  BAL, cytocentrifuge preparation, Wright/Giemsa
stain, light microscopy, MPV. Purulence, light. Blood,
moderate. Local materials, light. Enlarged pneumocyte with
intracytoplasmic inclusions. Morphology consistent with
cytomegalovirus. The large, regular-sized, magenta
cytoplasmic viral inclusions, when present, are characteristic
of this virus. Impression: cytomegalovirus disease.

Fig 7.   BAL, cytocentrifuge preparation,
immunofluorescent antibody adenovirus stain, fluorescence
microscopy, HPV. Prominent specific fluorescent staining of
infected cells. Impression: adenovirus infection. The necrosis
and cellular debris associated with adenovirus within the
bronchi can be easily overlooked because the necrotic,
virus-infected “smudge” cells may not be recognized.
Specific immunostaining should be performed on the basis
of clinical suspicion and compatible background material.

This is a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: http://amzn.to/2ctxo02