[Microbiology] Atlas of Direct examination in uncommon Gram-positive bacilli

Direct examination in uncommon Gram-positive bacilli, Atlas of Direct examination in uncommon Gram-positive bacilli, atlas in Microbiology, SUBCLINICAL ATLAS, MICROBIOLOGY ATLAS, tuyenlab.net, atlas in medical

Fig 1.  Abscess aspirate, smear, Gram stain, light
microscopy, HPV. Purulence, moderate. Amorphous debris;
light. Gram-positive bacilli, beaded. Suspect mycobacteria:
initiate additional testing (see Fig 2).

Fig 2.  Abscess aspirate, smear, acid-fast stain (ZiehlNeelsen),
light microscopy, MPV. Purulence, moderate.
Acid-fast bacilli, numerous. Mycobacterial cultures grew
Mycobacterium kansasii.

Fig 3.  Expectorated sputum, smear, Gram stain, light
microscopy, MPV. Amorphous debris, heavy. Bacillary shapes,
negative image. Oil removed from smear, decolorized with
acid alcohol, and immediately restained with Ziehl-Neelsen
acid-fast stain. Acid-fast bacilli, numerous. Specimen
recovered; acid-fast culture requested by laboratory.
Suspicious for tuberculosis.

Fig 4.  Expectorated sputum, concentrated smear,
fluorochrome acid-fast stain, fluorescent microscopy, MPV.
Typical acid-fast bacteria, numerous. Impression:
mycobacterial disease.
The patient had been placed in respiratory isolation
following physical examination and history and was
immediately begun on antituberculosis therapy following
receipt of the direct examination report. Mycobacterium
tuberculosis was identified from culture.

Fig 5.  Expectorated sputum, smear, Gram stain, light
microscopy, MPV. Purulence, light. Local materials, light.
Amorphous debris, moderate. Gram-positive bacilli, medium,
beaded. Follow-up acid-fast stain positive. Chest radiograph
with right upper lobe mass. Culture isolation of Rhodococcus
equi.

Fig 6.  Blood culture, sediment smear, Gram stain, light
microscopy, MPV. Blood. Gram-positive bacilli, branched,
beaded. Morphology consistent with Actinomyces or
Propionibacterium spp. Culture isolation of Actinomyces
israelii.

Fig 7.  Expectorated sputum, smear, Gram stain, light
microscopy, MPV. Purulence, light. Local materials, light.
Amorphous debris, moderate. Gram-positive bacilli,
branched, beaded. Morphotype consistent with Nocardia or
Actinomyces.

Fig 8.  Expectorated sputum, smear, partial acid-fast
stain, light microscopy, MPV. Partially acid-fast bacilli,
branched, beaded. Morphology consistent with Nocardia
spp. Impression: nocardiosis.

This is a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: http://amzn.to/2ctxo02