[Microbiology] Atlas of Anaerobes of Clinical Importance

Atlas of Anaerobes of Clinical Importance, Anaerobes of Clinical Importance, MICROBIOLOGY ATLAS, SUBCLINICAL ATLAS, atlas in microbiology, atlas in medical, tuyenlab.net, Connie R. Mahon, Donald C. Lehman and George Manuselis,

Fig 1. Classification of some clinically encountered clostridia by endospore
location. A, Gram-stained appearance of terminal spores of Clostridium tetani.
B, Gram-stained appearance of subterminal spores of Clostridium sordellii.

Fig 2. Gram-stained appearance of Actinomyces
israelii, illustrating the term Actinomyces-like.

Fig 3. Anaerobic specimen collection and transport
systems. Left: BBL Port-A-Cul with prereduced gel. Right: ESwab with
prereduced liquid Amies

Fig 4. Prereduced, anaerobically sterilized (PRAS)
plated media. PRAS plated media are manufactured,
packaged, shipped, and stored under anaerobic conditions.

Fig 5. Culture results that might be obtained from the primary isolation setup of a hypothetical “wound” specimen. This diagram illustrates the media and atmospheric conditions that would support growth of various organisms contained in the specimen. SBA, Blood agar; BBE, Bacteroides bile-esculin agar; BRU, brucella blood agar; CHOC, chocolate agar; KVLB, kanamycin-vancomycinlaked blood agar; MAC, MacConkey agar; PEA, phenylethyl
alcohol blood agar; CNA, Colistin nalidixic acid blood agar.

Fig 6. “Glove box” type of anaerobic chamber. The flexible, clear
vinyl chambers are available in three lengths (36, 59, and 78
inches), including one fitted with two pairs of gloves so that
two microbiologists can use the chamber simultaneously.

Fig 7. “Gloveless” type of anaerobic chamber with
dissecting microscope attachment. This stainless steel and
Plexiglas chamber is manufactured by Anaerobe Systems.

Fig 8. Anaerobic jars. This photograph depicts two
of the many different types of anaerobic jars that are
available commercially. As can be seen in this photograph,
the jars can also be used to culture microaerophilic
organisms.

Fig 9. Anaerobic pouch. This photograph depicts the
GasPak Pouch, one of the commercially available anaerobic
bag or pouch systems. Identification and susceptibility
testing systems requiring anaerobic incubation can be
incubated within some of the bags or pouches.

Fig 10. Anaerobic blood agar plate from an
intrauterine device culture as seen through a dissection
microscope. The heavy mixture of sizes and types of colonies
makes the task of isolating and identifying colonies very
difficult without the enhancement obtained with a
dissection microscope.

Fig 11. Schematic diagram for the initial identification of anaerobic isolates
based on Gram-stain morphology. Not all Clostridium spp. readily sporulate in clinical
specimens or in culture.

Fig 12. Examples of fluorescence observed with
long-wave ultraviolet light. A, Brick-red fluorescence
observed with Porphyromonas asaccharolytica. B, Chartreuse
fluorescence of Fusobacterium nucleatum.

Fig 13. Disks to add to the pure culture/subculture plate. SPS, Sodium
polyanethol sulfonate; V, vancomycin (5 µg); K, kanamycin (1000 µg); CL, colistin (10 µg).
Clostridium-like organisms are large, unbranched, gram-positive rods, with or without
spores. (Not all clostridia are large, and not all clostridia will stain as gram-positive rods.)

Fig 14. Typical special-potency antimicrobial disk
results for Clostridium ramosum: susceptible to vancomycin
(left) and kanamycin (right), and resistant to colistin (center
disk).

Fig 15. Positive lecithinase reaction on egg-yolk
agar. The reaction occurs within the agar. Clostridium
perfringens is shown here.

Fig 16. Positive lipase reaction on egg-yolk agar.
The reaction occurs on the surface of colonies and the
surrounding medium. A positive reaction by Fusobacterium
necrophorum is shown here.

Fig 17. Double zone of hemolysis produced by
Clostridium perfringens: inner zone of complete β-hemolysis
and outer zone of partial β-hemolysis.

Fig 18. Appearance of Bacteroides fragilis on a
kanamycin-vancomycin-laked blood agar (left) and
Bacteroides bile-esculin (BBE) agar biplate (right). Browning
of the BBE medium is the result of esculin hydrolysis.

Fig 19. Appearance of Bilophila wadsworthia on
BBE agar. Note the “fish-eye” appearance of the colonies.

Fig 20. Gram-stained appearance of Fusobacterium
nucleatum subsp. nucleatum, illustrating the fusiform
morphology of this organism.

Fig 21. RapID ANA-II preformed enzyme system. One of many systems available for
rapid definitive identification of commonly isolated
anaerobes.

Fig 22. Identification of Clostridium spp. Use this chart for organisms fulfilling
the following three criteria: (1) anaerobic, (2) gram-positive bacilli, and (3) spore formers.

Fig 23. Appearance of Actinomyces israelii showing
the “molar tooth” colonies typical for this anaerobe.

Fig 24. Gram-stained appearance of
Propionibacterium acnes, illustrating the term “diphtheroid.”

Fig 25. Gram-stained appearance of Bacteroides
thetaiotaomicron, illustrating the typical appearance of
Bacteroides spp.

Fig 26. Gram-stained appearance of Fusobacterium
mortiferum, illustrating pleomorphism.

Fig 27. Identification of anaerobic gram-positive cocci. Anaerobic gram-positive
cocci (AGPC) are susceptible to metronidazole, whereas microaerophilic gram-positive
cocci are not. An 80-µg metronidazole elution disk can be used to presumptively determine metronidazole susceptibility. Although metronidazole-resistant strains of AGPC have been reported, they appear to be rare. GLC, Gas-liquid chromatography; SPS, sodium polyanethol sulfonate.

Fig 28. Gram-stained appearance of a
Peptostreptococcus sp. illustrating the chain formation that
occurs with some species of anaerobic gram-positive cocci.

This is only a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: https://goo.gl/IawVC1