[Microbiology] Atlas of Mycoplasma and Ureaplasma

Mycoplasma and Ureaplasma, Atlas of Mycoplasma and Ureaplasma, atlas in medical, tuyenlab,net, Textbook of Diagnostic Microbiology 4th edition 2011, Connie R. Mahon, Donald C. Lehman and George Manuselis

Fig 1. Typical large Mycoplasma colony showing
“fried egg” appearance.

Fig 2. Electron micrographs showing effect of Mycoplasma pneumoniae on
ciliated tracheal cells. A, Infected animal model. B, Uninfected animal model.

Fig 3. Electron micrograph of Mycoplasma
pneumoniae attaching by specific attachment features to
ciliated trachea. mv, Microvilli; m, mycoplasma; c, cilia.

Fig 4. Typical chest radiograph of a patient with a
3-week course of atypical pneumonia. Note nonspecific
interstitial pneumonia and patchy infiltrate delineated by
feathery outline.

*SP4, Sucrose phosphate buffer, Mycoplasma base, fetal bovine serum (20%), phenol red. Medium stabilizes and decontaminates specimen. Storage at –70° C for repeat testing is recommended.
†Thin colony periphery. Examine with Stereomicroscope using 20× to 60× magnification.
‡Color change: positive, yellow color with no gross turbidity; negative, red color.
§GP–RBC–HAD = Guinea pig red blood cell hemadsorption. b-Hemolysis test for presumptive identification of Mycoplasma pneumoniae may be used in lieu of GP–RBC–HAD.
NOTE: Methylene blue on Dienes stain can be used for detection of Mycoplasma spp. on SP4 agar; plate immunofluorescence using labeled antibody can be used for identification.

Fig 5. Flow diagram for Mycoplasma spp. isolation using classic methods.

Fig 6. Dienes stain of Mycoplasma spp. colonies
demonstrating typical “fried-egg” appearance.

Fig 7. Typical mixed sizes of Mycoplasma spp. on
primary isolation media: Mycoplasma salivarium.

Fig 8. Identification of Mycoplasma-infected cell culture employing DNAfluorochrome stain (Hoechst no. 33258 stain). A, Mycoplasma orale. B, Uninfected Vero
cell culture highlighting the DNA-rich nucleus. C, Mycoplasma salivarium. The
mycoplasma appear as small pinpoint fluorescent bodies throughout the background.

Fig 9.  Mixed isolation of Mycoplasma hominis andUreaplasma urealyticum showing why U. urealyticum was
originally called “T” for “tiny-strain” (arrow).

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